Coding

Part:BBa_M11027:Design

Designed by: Trent Mortensen   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-17)

Cellobiohydrolase 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1260
    Illegal AgeI site found at 1336
    Illegal AgeI site found at 1411
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To avoid intron complications (as H. jecorina is a fungi), the amino acid sequence was copied from NCBI (protein P62694.1) and imported into the DNA 2.0 software. The sequence was then back-translated under E. coli optimization. There were Pst1 (ctgcag) sites at bp 67, which was mutated to ctgcaa, and at bp 1239, which was mutated to ctgctc. There were EcoR1 sites (gaattc) at bp 406, which was mutated to gaattt, and at bp 427, which was mutated to gaattt. As there were no stop codons on this modified sequence, two stop codons were added: taataa.

Source

Hyprocrea jecorina, anamorph: Trichoderma reesei

References